Here we provide an update on the new features offered in GenAlEx 6.5 that we believe will be welcomed by students, teachers and researchers. Moreover, the software has also attracted a large number of researchers who utilize its unique features. GenAlEx is now widely used by university teachers at both undergraduate and graduate levels around the world. Taking advantage of the rich graphical options available within Excel, GenAlEx offers a wide range of graphical outputs that aid genetic data analysis and interpretation. Packaging genetic analysis within a familiar and flexible environment resulted in quick understanding and effective performance of population genetic analyses. GenAlEx operates within Microsoft Excel-the widely used spreadsheet software that forms part of the cross-platform Microsoft Office suite. GenAlEx 6 was originally developed as a teaching tool to facilitate teaching population genetic analysis at the graduate level ( Peakall and Smouse, 2006). GenAlEx, and supporting documentation and tutorials are freely available at. The comprehensive guide has been fully revised.Īvailability and implementation: GenAlEx is written in VBA and provided as a Microsoft Excel Add-in (compatible with Excel 2003, 2007, 2010 on PC Excel 2004, 2011 on Macintosh). Teaching tutorials and expanded step-by-step output options are included. Export to more than 30 other data formats is provided. New features include calculation of new estimators of population structure: G′ ST, G′′ ST, Jost’s D est and F′ ST through AMOVA, Shannon Information analysis, linkage disequilibrium analysis for biallelic data and novel heterogeneity tests for spatial autocorrelation analysis. Both frequency-based ( F-statistics, heterozygosity, HWE, population assignment, relatedness) and distance-based (AMOVA, PCoA, Mantel tests, multivariate spatial autocorrelation) analyses are provided. GenAlEx offers analysis of diploid codominant, haploid and binary genetic loci and DNA sequences. Summary: GenAlEx: Genetic Analysis in Excel is a cross-platform package for population genetic analyses that runs within Microsoft Excel.
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